INSIGHTS
- Fungicide applied at R3 consistently increased soybean yield in the trial.
- Yield response to a fungicide applied at the V4 growth stage was minimal.
- Soybean varieties responded similarly to foliar fungicide applications.
Introduction
Foliar fungicide application timing in soybeans is a critical aspect of crop management that can significantly influence disease control efficacy and overall yield potential. Historically, the R3 growth stage (beginning pod) has been the optimal timing to apply fungicide (Figure 1). At this critical stage, soybeans are particularly vulnerable to various fungal pathogens. Applying fungicides at R3 maximizes spray coverage of the plant canopy, effectively protecting both existing and newly forming pods. This timing has consistently demonstrated yield improvements.
Figure 1. Soybeans at V4 growth stage on top and R3 growth stage on bottom from 2024 trials. Recent changes to soybean production including higher yield potential, earlier planting dates, rising disease pressure, and advancements in fungicide technology has prompted growers and agronomists to ask if there is a potential yield benefit to applying a fungicide earlier during vegetative growth stages.
Agronomy In Action Research Trial
In 2024, the Golden Harvest Agronomy in Action Research team implemented a trial at six locations across Illinois, Iowa, Kansas, Nebraska and Wisconsin to evaluate the effect of a fungicide application during the V4 growth stage (four unfolded trifoliate leaves, Figure 1). The fungicide application at V4 was compared to a traditional application timing at R3 and a no fungicide or untreated check. A combination treatment of a fungicide applied at V4 and R3 was also included. All fungicide applications used Miravis® Neo fungicide applied at 13.7 oz/acre.
- Check – no fungicide applied
- V4 Fungicide
- R3 Fungicide
- V4 + R3 Fungicide
Four soybean varieties were planted at each location depending on geography (Table 1).
Table 1. Variety groups planted at each location based on relative maturity (RM). Yield Results
Despite environmental conditions conducive for disease for much of the growing season, overall disease pressure was low at all sites. Sclerotinia stem rot (white mold) was present at Malta, IL and was likely the reason soybean yields were lower there. Janesville, WI had septoria brown spot but symptoms did not progress up the canopy.
Yield environments ranged from 66 Bu/A at Malta, IL to 87 Bu/A at Clinton, IL. There was no interaction between variety and fungicide treatment, so all results are averaged across varieties. On average across all locations, fungicide applied at the R3 growth stage significantly increased yield by 2.5 Bu/A compared to the untreated check (Graph 1). Fungicide at R3 statistically increased yield at 2 out of the 6 locations with a 3.8 Bu/A response at Clay Center, KS and 4.5 Bu/A response at Malta, IL. Yield response to R3 fungicide applications ranged from 1.5 – 4.5 Bu/A depending on the location.
Fungicide application at V4 did not significantly increase yield at any location. Clinton, IL (1.7 Bu/A) and Janesville, WI (1.0 Bu/A) were the only locations with a numerical response of at least 1 Bu/A (Graph 1). On average across all locations, combining V4 and R3 fungicide timings did not out yield R3 application alone (Graph 1). Although not statistically different, only Janesville, WI, observed greater yields with two application timings compared to the R3 timing alone.
Graph 1. Soybean yield response to foliar fungicide timing at six locations averaged across four varieties in 2024. Summary
Results from this study confirm the consistency in yield response from R3 fungicide applications and why it should be considered as part of a comprehensive soybean management program. Even in environments with low disease pressure, positive responses to fungicide at the R3 timing was observed. In contrast, fungicide applied during the vegetative stage of V4 had minimal effect on yield. There may be environments and situations where an early season fungicide application is necessary to prevent heavy disease pressure, however this timing should not be a widely used management strategy.
Fungicide application timing in soybeans is critical and the R3 growth stage continues to be the optimal timing to maximize spray coverage and protect soybeans from disease at the most vulnerable period in their life cycle.
All photos are either the property of Syngenta or are used with permission.
Product performance assumes disease presence.
© 2025 Syngenta. Important: Always read and follow label instructions. Some products may not be registered for sale or use in all states or counties. Please check with your local extension service to ensure registration status. AAtrex 4L, AAtrex Nine-O, Acuron, Agri-Flex, Agri-Mek 0.15 EC, Agri-Mek SC, Avicta 500FS, Avicta Complete Beans 500, Avicta Complete Corn 250, Avicta Duo 250 Corn, Avicta Duo Corn, Avicta Duo COT202, Avicta Duo Cotton, Besiege, Bicep II Magnum, Bicep II Magnum FC, Bicep Lite II Magnum, Callisto Xtra, Denim, Endigo ZC, Endigo ZCX, Epi-Mek 0.15EC, Expert, Force, Force 3G, Force CS, Force 6.5G, Force Evo, Gramoxone SL 2.0, Gramoxone SL 3.0, Karate, Karate with Zeon Technology, Lamcap, Lamcap II, Lamdec, Lexar EZ, Lumax EZ, Medal II ATZ, Minecto Pro, Proclaim, Voliam Xpress and Warrior II with Zeon Technology are Restricted Use Pesticides.
Some seed treatment offers are separately registered products applied to the seed as a combined slurry. Always read individual product labels and treater instructions before combining and applying component products. Orondis Gold may be sold as a formulated premix or as a combination of separately registered products: Orondis Gold 200 and Orondis Gold B.
No dicamba may be used in-crop with seed with Roundup Ready® Xtend Technology, unless and until approved or specifically permitted, and no dicamba formulations are currently registered for such use at the time this material was published. Please follow https://www.roundupreadyxtend.com /pages/xtendimax-updates.aspx for status updates. See product labels for details and tank mix partners. Golden Harvest® and NK® soybean varieties are protected under granted or pending U.S. variety patents and other intellectual property rights, regardless of the trait(s) within the seed. The Enlist E3® soybean, LibertyLink®, LibertyLink® GT27®, Roundup Ready 2 Xtend®, Roundup Ready 2 Yield® and XtendFlex® soybean traits may be protected under numerous United States patents. It is unlawful to save soybeans containing these traits for planting or transfer to others for use as a planting seed. NOT ALL formulations of dicamba, glyphosate or glufosinate are approved for in-crop use with products with XtendFlex® Technology. ONLY USE FORMULATIONS THAT ARE SPECIFICALLY LABELED FOR SUCH USES AND APPROVED FOR SUCH USE IN THE STATE OF APPLICATION. Contact the U.S. EPA and your state pesticide regulatory agency with any questions about the approval status of dicamba herbicide products for in-crop use with products with XtendFlex® Technology. Only 2,4-D choline formulations with Colex-D® Technology are approved for use with Enlist E3® soybeans. ENLIST E3® soybean technology is jointly developed with Corteva Agriscience LLC and M.S. Technologies, L.L.C. The ENLIST trait and ENLIST Weed Control System are technologies owned and developed by Corteva Agriscience LLC. ENLIST® and ENLIST E3® are trademarks of Corteva Agriscience LLC. GT27® is a trademark of M.S. Technologies, L.L.C. and BASF. Roundup Ready 2 Xtend®, Roundup Ready 2 Yield® and XtendFlex® are registered trademarks used under license from the Bayer Group.
Trademarks are the property of their respective owners.